Journal: mSphere
Article Title: Genetic Determinants of Resistance to Extended-Spectrum Cephalosporin and Fluoroquinolone in Escherichia coli Isolated from Diseased Pigs in the United States
doi: 10.1128/mSphere.00990-20
Figure Lengend Snippet: Characteristics of isolates carrying mcr-9 genes
Article Snippet: The aim of the present study was to identify and characterize the genetic basis of resistance against fluoroquinolones (enrofloxacin) and extended-spectrum cephalosporins (ceftiofur) in swine E. coli isolates using whole-genome sequencing (WGS). bla CMY-2 (carried by IncA/C2, IncI1, and IncI2 plasmids), bla CTX-M (carried by IncF, IncHI2, and IncN plasmids), and bla SHV-12 (carried by IncHI2 plasmids) genes were present in 87 (82.1%), 19 (17.9%), and 3 (2.83%) of the 106 ceftiofur-resistant isolates, respectively.
Techniques: Plasmid Preparation